Semi Prep HPLC Column, Diamond Hydride, 4 um, 100 A. 10 mm ID x 150 mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 70000‑SP150 Diamond Hydride Semi‑Preparative HPLC Column is intended for advanced scale‑up and purification workflows where analytical‑scale columns do not provide sufficient loading capacity or resolution. This longer 150 mm configuration delivers substantially increased resolving power for difficult separations while allowing collection of milligram‑level quantities of target compounds.
Semi‑Preparative Use Case: Why 10 mm ID × 150 mm
The 10 mm internal diameter places this column squarely in the semi‑preparative class, making it ideal for laboratories scaling methods from 4.6 mm analytical columns without moving directly to full preparative systems.
Compared to shorter semi‑prep formats, the 150 mm bed length provides:
Significantly higher theoretical plate count
Improved separation of closely eluting or structurally similar analytes
Increased confidence during fraction collection
Better alignment with resolution‑limited analytical methods
This format is selected when resolution is the dominant requirement in semi‑prep purification.
Diamond Hydride™ TYPE‑C Stationary Phase Advantages
Manufactured with Cogent TYPE‑C™ silica hydride technology, the Diamond Hydride stationary phase offers clear advantages over conventional bonded‑silica HILIC phases for semi‑prep work:
Stable and reproducible retention for polar and moderately polar analytes
Faster equilibration, reducing cycle time between injections
Robust column lifetime, even under high‑organic mobile‑phase conditions
This chemistry is especially effective for purification of:
Peptides and peptide‑like compounds
Metabolites and highly polar small molecules
Analytes containing amine or basic functional groups
Other compounds best resolved using HILIC‑based separations
Purpose‑Built for Semi‑Prep Scale‑Up
The 70000‑SP150 enables predictable scale‑up from analytical HILIC methods while maintaining chromatographic behavior. It bridges method development and larger‑scale purification without the complexity or solvent demands of full preparative hardware.
System Requirements
Typical operating flow rates: ~3–8 mL/min
Requires a semi‑preparative HPLC system capable of:
Higher flow delivery
Larger injection volumes
Appropriate detector configuration and fraction collection
Standard analytical HPLC systems are not suitable for 10 mm ID semi‑preparative columns.
Flow rates may be optimized further based on mobile‑phase viscosity, pressure limits, and purification objectives.
Made‑to‑Order Columns & Pricing Information
These columns represent a significant investment and are often application‑specific. To ensure customers receive the correct column for their workflow:
Pricing and technical support is provided through our Customer Service Department via formal quotation. Use our online form to start the process or call customer service.
Columns are produced on order, based on customer requirements
Orders cannot be returned or canceled once placed